The Third Generation Anti-HER2 Chimeric Antigen Receptor Mouse T Cells Alone or Together With Anti-PD1 Antibody Inhibits the Growth of Mouse Breast Tumor Cells Expressing HER2 in vitro and in Immune Competent Mice
Chimeric Antigen Receptor (CAR) T cells have great efficacy against CD19 + leukemia but little success to solid tumors. This study explored the effectiveness of anti-HER2 cells CAR-T third generation alone or in combination with anti-PD1 antibody to HER2 expressing breast tumor cells in vitro and in a mouse model of immune competent. Milk mouse cell line 4T1 tumor-positive PDL1 engineered to express luciferase and human HER2 is used as the target cell line (4T1-Luc-HER2). anti-HER2 cells CAR-T produced by transducing rat spleen T cells with recombinant lentivirus. ELISA analysis showed that IL-2 and IFN-γ secretion is increased in CAR-T cells co-cultured with target cells, and the secretion of two cytokines is increased further by the addition of anti-PD1 antibodies. Lactate dehydrogenase assay revealed that the CAR-T cells displayed potent cytotoxicity against target cells, and the addition of anti-PD1 antibody further enhanced the cytotoxicity.
In the effector: target ratio of 16: 1, cytotoxicity was 39.8% with CAR-T cells alone, and increased to 49.5% with the addition of anti-PD1 antibodies. In syngeneic rat model of immune competent, CAR-T cells were found to be present in the stroma of tumors, inhibiting tumor growth and increased apoptosis of tumor significantly. Besides anti-PD1 antibody further enhanced anti-tumor activity of this. Twenty-one days after treatment, tumor weight was reduced to 50.0% and 73.3% in the CAR-T group and CAR-T plus anti-PD1 group compared with the group T is empty. Our results indicate that the anti-PD1 antibodies can greatly enhance the effectiveness of anti-HER2 CAR-T against HER2-positive solid tumors.
paraneoplastic neurological syndromes (PNS) well established business entities associated with the antibody onconeural. paraneoplastic parkinsonism is presenting a very under reported from the PNS. the rapid development of typical or atypical parkinsonism with red flags such as the involvement of multiaxial, complete staring palsy and Clinico-radiological incompatibility should be a doctor for a suspect secondary etiology such as infection, metabolic, vascular, metastasis and paraneoplastic causes. We describe a patient with rapidly progressive parkinsonism. The diagnosis of breast carcinoma associated ANNA-2 / Anti Ri made mediated PSP like phenotype in these patients based on clinical examination and investigation.
The Third Generation Anti-HER2 Chimeric Antigen Receptor Mouse T Cells Alone or Together With Anti-PD1 Antibody Inhibits the Growth of Mouse Breast Tumor Cells Expressing HER2 in vitro and in Immune Competent Mice
A Plant-Derived Antigen-Antibody Responses Complex Anti-Cancer Induces Immune by Quaternary Structure Forming Large
Antigen-antibody complexes (AAC) have a new function for immunomodulation, encourage the implementation of various quaternary structure of proteins for vaccination. In this study, GA733 antigen and antibody anti-GA733 proteins both co-expressed to obtain protein structures AAC in an F1 plant obtained by crossing plants expressing each protein. F1 plants, antigen and antibody quaternary structure are assembled to form a large circular ACC (~ 30 nm).
Description: Recombinant Interleukin-15 is a disulfide-linked homodimeric protein consisting of two 115 amino acid residues, and migrates as an approximately 13 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-15 mature chain was expressed in E. coli.
Description: Recombinant Interleukin-15 is a disulfide-linked homodimeric protein consisting of two 115 amino acid residues, and migrates as an approximately 13 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-15 mature chain was expressed in E. coli.
Description: Quantitativesandwich ELISA kit for measuring Human Interleukin 15, IL-15 in samples from serum, plasma, tissue homogenates, cell culture supernates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Interleukin 15, IL-15 in samples from serum, plasma, tissue homogenates, cell culture supernates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Interleukin-15 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids (and an N-terminal Methionine) and having a molecular mass of 12.8kDa. The IL-15 is purified by proprietary chromatographic techniques.
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages.
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: The 15-414 monoclonal antibody binds with human CD172a, a 85 - 90 kD glycoprotein often found on the surface of dendritic cells and neurons.
Description: Antibody raised against 15 Lipoxygenase 2
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Quaternary protein structure caused anticancer immune response to generate immunoglobulin G (IgG) specific to the corresponding antigen in mice. Serum containing IgG anticancer inhibit human colorectal cancer cell growth in nude mouse xenografts. Taken together, the antigen and antibody can be assembled to form the structure of AAC proteins in plants. Plants intersection is an alternative strategy for the establishment of an efficient vaccine AAC increase production of anticancer antibodies.
Chris
