Eplet Mismatch Load and De Novo Occurrence of Donor-Specific Anti-HLA Antibodies, Rejection, and Graft Failure after Kidney Transplantation: An Observational Cohort Study
Background: In kidney transplantation, evaluating HLA mismatches eplets-small patch of surface-exposed amino acids of the HLA molecule-antigen mismatch may not offer a better approach for assessing donor-recipient HLA mismatch and increasing the risk assessment and prediction of the results of transplantation.
Methods: To evaluate the effect of the number of mismatches Eplet (load mismatch) on the formation of de novo antibody donor specific HLA (DSA) and the results of transplantation, we conducted a cohort study which included recipients of renal grown successively transplanted in one center from March 2004 to February 2013 . We conducted a retrospective high resolution genotyping HLA loci of 926 pairs of transplantation and the use of computer algorithms to calculate HLAMatchmaker Eplet HLA mismatches.
Result: De novo DSAs occurred in 43 (4.6%) patients. Multivariable analysis showed a significant independent association between antibody-verified Eplet load mismatch and de novo DSA events and graft failure, mainly explained by DQ antibody Eplet effect is verified. Association with DQ antibody-verified discrepancy Eplet is linear, indefinitely safe where de novo DSA does not occur. Odds for T cell- or antibody-mediated rejection increased by 5% and 12%, respectively, per antibody-verified Eplet DQ mismatch.
Conclusion: Eplet mismatches in HLA-DQ confer substantial risk for de novo formation of DSA, graft rejection, and graft failure after kidney transplantation. Mismatches in other loci seem to have less effect. The results showed that HLA-DQ antibody Eplet mismatch load-verified personal can use to guide post-transplant immunosuppression. Adoption molecular match for DQA1 and DQB1 alleles can also help to minimize the de novo formation of DSA and potentially improve transplant outcome.
Eplet Mismatch Load and De Novo Occurrence of Donor-Specific Anti-HLA Antibodies, Rejection, and Graft Failure after Kidney Transplantation: An Observational Cohort Study
Anti-Cancer Effect of 3-Hydroxy-β-Ionone Identified from Moringa oleifera Lam. Man leaves 15 squamous cell carcinoma cells
Squamous cell carcinoma is the most common type of cancer worldwide head and neck. Radiation and chemotherapy common treatment for patients; However, these drugs can have side effects and tumors develop drug resistance. effective treatments still require improvements for cancer patients. Here, we examined the effects of anti-cancer properties of Moringa oleifera (MO) Lam. leaf extract and its fractions, 3-hydroxy-β-ionone in SCC15 cells. SCC15 treated with and without MO leaf extract and its fractions.
MTT assay was used to determine cell viability in SCC15. cell cycle and apoptosis was evaluated by Muse ™ cell analyzer. colony formation and wound closure SCC15 analysis conducted in 6-well plates. Apoptotic markers were evaluated by immunoblotting. We found that an extract of moringa and 3-HBI significantly inhibit proliferation of SCC15. In addition, they induced apoptosis and cell cycle arrest in G2 / M phase in SCC15 compared with untreated controls. MO extract and 3-HBI also inhibit the colony formation and cell migration SCC15.
In addition, we observed upregulation of cleaved caspase-3 and Bax with downregulation of anti-apoptotic Bcl-2, which shows the induction of apoptosis of cancer cells. Our results show that extracts of MO and 3-HBI supplied anti-cancer properties by inhibiting the progression and induce apoptosis of SCC15.
Description: This monoclonal antibody is for studies of antigen expression in cells and tissue sections using immunocytochemistry and immunoprecipitation
Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Galectin-1 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with β-galactoside moieties. Galectin-1 is an animal lectin that has been shown to interact with CD3, CD4, and CD45. It induces apoptosis of activated T-cells and T-leukemia cell lines and inhibits the protein phosphatase activity of CD45. Recombinant human Galectin-1 is a 14.5 kDa protein containing 134 amino acid residues.
Description: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creatingimmune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.
Description: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.
Description: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1) in serum, plasma and other biological fluids.
Description: Quantitativesandwich ELISA kit for measuring Human Galectin-1 (LGALS1) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Galectin-1(LGALS1) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive ELISA for quantitative measurement of Human Galectin-1(LGALS1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Galectin-1(LGALS1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Galectin-1(LGALS1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich quantitative ELISA assay kit for detection of Human Galectin 1 (GAL1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Galectin 1 (GAL1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 1 (GAL1) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 1 (GAL1) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 1 (GAL1) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 1 (GAL1) in serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Galectin 1 (GAL1) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Quantitative sandwich ELISA for measuring Human Galectin-1 (LGALS1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Galectin-1 (LGALS1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Galectin-1 (LGALS1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: A sandwich ELISA kit for detection of Galectin 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: LGALS1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 135 amino acids and having a molecular mass of 14.7kDa.;The LGALS1 is purified by proprietary chromatographic techniques.
Human Galectin-1 (Gal-1) AssayLite Antibody (FITC Conjugate)
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Galectin-1 . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Galectin-1 . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody for detection of Galectin-1 from Human, Rat. This Galectin-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Galectin-1 at AA rangle: 10-90
Description: A polyclonal antibody for detection of Galectin-1 from Human, Rat. This Galectin-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Galectin-1 at AA rangle: 10-90
Description: A polyclonal antibody for detection of Galectin-1 from Human, Rat. This Galectin-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Galectin-1 at AA rangle: 10-90
Description: A polyclonal antibody for detection of Galectin-1 from Human, Mouse, Rat. This Galectin-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Galectin-1 protein at amino acid sequence of 30-80
Description: A polyclonal antibody for detection of Galectin-1 from Human, Mouse, Rat. This Galectin-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Galectin-1 protein at amino acid sequence of 30-80
Description: A polyclonal antibody for detection of Galectin-1 from Human, Mouse, Rat. This Galectin-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Galectin-1 protein at amino acid sequence of 30-80
Description: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
Description: Galectin 1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kDa participating in a variety of normal and pathological processes, including cancer progression. Galectin 1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin 1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin 1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.
Description: Galectin 1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kDa participating in a variety of normal and pathological processes, including cancer progression. Galectin 1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin 1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin 1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.
Description: Galectin 1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kDa participating in a variety of normal and pathological processes, including cancer progression. Galectin 1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin 1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin 1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.
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Hesperidin hydrolyzate (HHS) is produced by the hydrolysis of hesperidin (HDN) in the previous study. Potential component in HHS identified by LC-MS, and the minor component (MCS) in HHS isolated. Antioxidant activity with the capacity of the radical-scavenging capacity reduces test and β-carotene-linoleic, anti-inflammatory effects by inhibiting the production of NO from RAW 264.7 cells, and α-glucosidase inhibitory effects of HDN, HHS, MCS and henperetin (HTN) was investigated in the research this
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