ackground: osteoarthritis is a leading cause of disability worldwide; cartilage degeneration and disability is the central feature. significant advances in tissue engineering holds the promise to regenerate damaged cartilage tissue. However, the formidable challenge is to develop a network construction of three-dimensional (3D) that can regulate the local immune environment to facilitate osteochondral intrinsic regeneration. Objective: This study was to evaluate the efficacy of a 3D-printed decellularized cartilage extracellular matrix (ECM) and polyethylene glycol diakrilat (PEGDA) integrates novel scaffold (PEGDA / ECM) together with honokiol natural compounds (Hon) for regeneration of the defect osteochondral.
Study Design: Controlled laboratory study.
Methods: We used a 3D printer based stereolithography to PEGDA / ECM bioprinting. A total of 36 Sprague-Dawley rats with a cylindrical osteochondral defect in the groove trochlear femur were randomized into three different treatment: no implantation of the scaffold (Defect group), the 3D printed PEGDA / ECM scaffold alone (PEGDA / group ECM), or Hon depends on a 3D-printed scaffold PEGDA / ECM (PEGDA / ECM / hon group). 12 rats that underwent surgery incision medial parapatellar only be used as normal controls. Postoperative femur specimens were harvested at 4 and 8 weeks for gross, micro-CT, and histological evaluation. Efficacy PEGDA / ECM / Hon scaffolding on the release of proinflammatory cytokines from macrophages stimulated by lipopolysaccharide (LPS) was evaluated in vitro.
Results: In-vitro results determined that PEGDA / ECM / Hon scaffold can suppress the release of proinflammatory cytokines from macrophages stimulated by LPS. Macroscopic picture shows that / Hon groups PEGDA / ECM has ICRS scored significantly higher than the defect and PEGDA / ECM group. Micro-CT evaluation showed that much thinner tissue formed at the defect site implanted with scaffolds PEGDA / ECM or PEGDA / ECM / Hon scaffold compared with untreated defects. Histological analysis showed that the group PEGDA / ECM / Hon had a significant increase in the regeneration of osteochondral at 4 and 8 weeks after surgery compared with ECM / PEGDA or disabled people.
Conclusion: This study shows that 3D printing of PEGDA / ECM hydrogels combine anti-inflammatory phytomolecule honokiol could provide a promising scaffold for osteochondral defect repair.
3D-Printed Extracellular Matrix/Polyethylene Glycol Diacrylate Hydrogel Incorporating the Anti-inflammatory Phytomolecule Honokiol for Regeneration of Osteochondral Defects
Bee venom exerts systemic anti-arthritic and anti-inflammatory properties in a mouse model of arthritis
Bee venom (BV) is widely used as a traditional Chinese medicine to treat a variety of conditions, including rheumatoid arthritis (RA). The purpose of this study was to evaluate the effect of systemic BV (60 mg / kg) as a natural anti-rheumatic products, compared with methotrexate and identify the underlying mechanisms of action BV possibility of using an adjuvant-induced arthritis rat complete Freund.
The development of signs of RA signs (knee circumference joints and arthritis index scores) were evaluated. erythrocyte sedimentation rate, serum tumor necrosis factor-α (TNF-α) and serum interleukin-1β (IL-1β) levels were measured at the end of the study. Histopathological examination followed by immunostaining of NF-kB (P65) performed on the affected knee joints. In addition, in vitro cyclooxygenase (COX) inhibition activity, carrageenan foot edema test and acetic acid writhing test was performed to evaluate the effect of anti-inflammatory and analgesic doses were assessed and compared with diclofenac.
Alpha V Beta 5 Integrin, Peptide Aptamer, Biotinylated
Description: Integrin alpha-5 belongs to the Integrin alpha chain family and contains 7 FG-GAP repeats. Integrin alpha-5 joins with Integrin- beta 1 to form a fibronectin and laminin receptor which recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. It is expressed on fibroblasts, endothelial cells, peripheral T cells and platelets. Integrin alpha-5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains. In addition to adhesion, ITGA5 participates in cell-surface mediated signalling.
Description: Integrin alpha5 is a protein encoded by the ITGA5 gene which is approximately 114,5 kDa. Integrin alpha5 is localised to the cell membrane and is involved in the MAPK-Erk pathway, apoptotic pathways, integrin pathway, focal adhesion and blood-brain barrier and immune cell transmigration. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signalling. Integrin alpha5 is expressed in the liver, lung, nervous system, bone marrow and blood. Mutations in the ITGA5 gene may result in colon carcinoma and congenital epulis STJ97284 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Integrin alpha5 protein.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Integrin Alpha 5 (ITGa5) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5 (ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5(ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Integrin alpha-5 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: A sandwich ELISA kit for detection of Integrin Alpha 5 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Integrin alpha-5 in samples from serum, plasma, tissue homogenates and other biological fluids.
An acute toxicity test conducted to establish security BV at high doses. The results of this study highlight the potential for systemic BV to prevent the development of signs of RA. BV also significantly reduce the serum levels of TNF-α, IL-1β and NF-kB in the affected joint
Chris
