ackground: osteoarthritis is a leading cause of disability worldwide; cartilage degeneration and disability is the central feature. significant advances in tissue engineering holds the promise to regenerate damaged cartilage tissue. However, the formidable challenge is to develop a network construction of three-dimensional (3D) that can regulate the local immune environment to facilitate osteochondral intrinsic regeneration. Objective: This study was to evaluate the efficacy of a 3D-printed decellularized cartilage extracellular matrix (ECM) and polyethylene glycol diakrilat (PEGDA) integrates novel scaffold (PEGDA / ECM) together with honokiol natural compounds (Hon) for regeneration of the defect osteochondral.
Study Design: Controlled laboratory study.
Methods: We used a 3D printer based stereolithography to PEGDA / ECM bioprinting. A total of 36 Sprague-Dawley rats with a cylindrical osteochondral defect in the groove trochlear femur were randomized into three different treatment: no implantation of the scaffold (Defect group), the 3D printed PEGDA / ECM scaffold alone (PEGDA / group ECM), or Hon depends on a 3D-printed scaffold PEGDA / ECM (PEGDA / ECM / hon group). 12 rats that underwent surgery incision medial parapatellar only be used as normal controls. Postoperative femur specimens were harvested at 4 and 8 weeks for gross, micro-CT, and histological evaluation. Efficacy PEGDA / ECM / Hon scaffolding on the release of proinflammatory cytokines from macrophages stimulated by lipopolysaccharide (LPS) was evaluated in vitro.
Results: In-vitro results determined that PEGDA / ECM / Hon scaffold can suppress the release of proinflammatory cytokines from macrophages stimulated by LPS. Macroscopic picture shows that / Hon groups PEGDA / ECM has ICRS scored significantly higher than the defect and PEGDA / ECM group. Micro-CT evaluation showed that much thinner tissue formed at the defect site implanted with scaffolds PEGDA / ECM or PEGDA / ECM / Hon scaffold compared with untreated defects. Histological analysis showed that the group PEGDA / ECM / Hon had a significant increase in the regeneration of osteochondral at 4 and 8 weeks after surgery compared with ECM / PEGDA or disabled people.
Conclusion: This study shows that 3D printing of PEGDA / ECM hydrogels combine anti-inflammatory phytomolecule honokiol could provide a promising scaffold for osteochondral defect repair.
Bee venom exerts systemic anti-arthritic and anti-inflammatory properties in a mouse model of arthritis
Bee venom (BV) is widely used as a traditional Chinese medicine to treat a variety of conditions, including rheumatoid arthritis (RA). The purpose of this study was to evaluate the effect of systemic BV (60 mg / kg) as a natural anti-rheumatic products, compared with methotrexate and identify the underlying mechanisms of action BV possibility of using an adjuvant-induced arthritis rat complete Freund.
The development of signs of RA signs (knee circumference joints and arthritis index scores) were evaluated. erythrocyte sedimentation rate, serum tumor necrosis factor-α (TNF-α) and serum interleukin-1β (IL-1β) levels were measured at the end of the study. Histopathological examination followed by immunostaining of NF-kB (P65) performed on the affected knee joints. In addition, in vitro cyclooxygenase (COX) inhibition activity, carrageenan foot edema test and acetic acid writhing test was performed to evaluate the effect of anti-inflammatory and analgesic doses were assessed and compared with diclofenac.
Description: Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5 (ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5(ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Integrin Alpha 5 (ITGa5) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITGa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITGa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITGa5 in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITGa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITGa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITGa5 in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].
Description: Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Description: A sandwich ELISA kit for detection of Integrin Alpha 5 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
CD49e, CT (ITGA5, FNRA, Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e, Integrin alpha-5 heavy chain, Integrin alpha-5 light chain)
An acute toxicity test conducted to establish security BV at high doses. The results of this study highlight the potential for systemic BV to prevent the development of signs of RA. BV also significantly reduce the serum levels of TNF-α, IL-1β and NF-kB in the affected joint